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    當前位置 : Millipore >>> Millipore/ABS82 | Anti-VEGF Antibody/ABS82/100 µg
    Millipore/ABS82 | Anti-VEGF Antibody/ABS82/100 µg
    • Millipore/ABS82 | Anti-VEGF Antibody/ABS82/100 µg

    Millipore/ABS82 | Anti-VEGF Antibody/ABS82/100 µg

    價格: ¥3444.00 市場價: 5740.00

    貨號: ABS82
    品牌: Millipore
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      • Description
        CatalogueNumberABS82
        Replaces07-1376
        DescriptionAnti-VEGFAntibody
        AlternateNames
        • vascularendothelialgrowthfactorA
        • vascularendothelialgrowthfactor
        • VascularpermeABIlityfactor
        • vascularendothelialgrowthfactorisoformVEGF
        BackgroundInformationVEGF(Vascularendothelialgrowthfactor,VEGFA,VEGF-A),adimericligand,isamongthemostpotentangiogenicmitogens.VEGFissecretedbytumorcellsandothercellsexposedtohypoxia.VEGFisahighlyspecificmitogenforvascularendothelialcells.SevenVEGFisoforms(a,b,c,d,e,f,g)aregeneratedasaresultofalternativesplicingfromasingleVEGFgene.ThemostcommonlystudiedisoformsareVEGF121,VEGF165,andVEGF189(f,dandb,respectively)AllsevenisoformsdifferintheirmolecularmassandinBIOLOGicalpropertiessuchastheirabilitytobindtocell-surfaceheparan-sulfateproteoglycans.TheexpressionofVEGFispotentiatedandissecretedbytumorcellsinresponsetohypoxia,byactivatedoncogenes,andbyavarietyofcytokines.VEGFinducesangiogenesisaswellaspermeabilizationofbloodvessels,andplaysacentralroleintheregulationofvasculogenesis.VEGFexpressioncorrelatestothedegreeoftumorvascularizationandwithincreasedmetastaticrisk.Additionally,VEGFinducesendothelialcellproliferation,promotescellmigration,andinhibitsapoptosis.Consequently,inhibitionofVEGFsignalingabrogatesthedevelopmentofawidevarietyoftumors.
        ProductInformation
        FormatAffinityPurified
        Control
        • HuVeccelllysate
        PresentationPurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
        Applications
        ApplicationDetectVEGFusingthisAnti-VEGFAntibodyvalidatedforuseinWB,IH(P).
        KeyApplications
        • WesternBlotting
        • Immunohistochemistry(Paraffin)
        ApplicationNotesImmunohistochemistryAnalysis:1:400dilutionfromapreviouslotdetectedVEGFinnormalhumanplacentalvillitissue.
        BiologicalInformation
        ImmunogenKLH-conjugatedlinearpeptidecorrespondingtohumanVEGFattheN-terminus.
        EpitopeN-terminus
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostRabbit
        SpecificityThisantibodyrecognizesVEGFattheN-terminus.
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        • Primate
        SpeciesReactivityNoteDemonstratedtoreactwithhuman,mouse,andrat.Predictedtoreactwithprimatebasedon100%sequencehomology.
        Otherhomologies:Bovine(85%sequencehomology).Rat(70%sequencehomology).Mouse(65%sequencehomology).
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryThisgeneisamemberofthePDGF/VEGFgrowthfactorfamilyandencodesaproteinthatisoftenfoundasadisulfidelinkedhomodimer.Thisproteinisaglycosylatedmitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeability,inducingangiogenesis,vasculogenesisandendothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.ElevatedlevelsofthisproteinislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.Alternativelysplicedtranscriptvariants,encodingeitherfreelysecretedorcell-associatedisoforms,havebeencharacterized.Thereisalsoevidencefortheuseofnon-AUG(CUG)translationinitiationsitesupstreamof,andin-framewiththefirstAUG,leADIngtoadditionalisoforms.[providedbyRefSeq].
        GeneSymbol
        • VPF
        • VEGF-A
        • MVCD
        • VEGF
        • VEGA
        PurificationMethodAffinityPurfied
        UniProtNumber
        UniProtSummaryFUNCTION:Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145.

        SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity).

        SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin.

        ALTERNATIVEPRODUCTS:10namedisoforms[FASTA]producedbyalternativesplicing.
        MolecularWeight~55kDaobserved.UniProtKB/Swiss-ProtP15692(VEGFA_HUMAN)entrydescribes13isoformsproducedbyalternativepromoterusage,alternativesplicingandalternativeinitiation:Isoform1at27.042kDa,Isoform2at25.173kDa,Isoform3at24.422kDa,Isoform4at22.314kDa,Isoform5at20.218kDa,Isoform6at20.064kDa,Isoform7at22.259kDa,Isoform8at17.219kDa,Isoform9at15.981kDa,Isoform10at40.738kDa,Isoform11at35.643kDa,Isoform12at43.597kDa,Isoform13at45.467kDa.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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