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    當前位置 : Millipore >>> Millipore/ABS44 | Anti-MAPK 1/2 Antibody/ABS44/100 µg
    Millipore/ABS44 | Anti-MAPK 1/2 Antibody/ABS44/100 µg
    • Millipore/ABS44 | Anti-MAPK 1/2 Antibody/ABS44/100 µg

    Millipore/ABS44 | Anti-MAPK 1/2 Antibody/ABS44/100 µg

    價格: ¥3324.00 市場價: 5540.00

    貨號: ABS44
    品牌: Millipore
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      • Description
        CatalogueNumberABS44
        Replaces06-182
        DescriptionAnti-MAPK1/2Antibody
        AlternateNames
        • ERK-1
        • ERT2
        • Extracellularsignal-regulatedkinase1
        • Insulin-stimulatedMAP2kinase
        • MAPkinase3
        • MAPkinaseisoformp44
        • MAPK3
        • Microtubule-associatedprotein2kinase
        • Mitogen-activatedproteinkinase3
        • MNK1
        • p44-ERK1
        • p44-MAPK
        • ERK-2
        • ERT1
        • Extracellularsignal-regulatedkinase2
        • MAPkinase1
        • MAPkinase2
        • MAPkinaseisoformp42
        • MAPK1
        • MAPK2
        • Mitogen-activatedproteinkinase1
        • Mitogen-activatedproteinkinase2
        • p42-MAPK
        BackgroundInformationMitogen-activatedproteinkinase3(EC2.7.11.24;UniProtP21708;alsoknownasERK-1,ERT2,Extracellularsignal-regulatedkinase1,Insulin-stimulatedMAP2kinase,MAPkinase3,MAPkinaseisoformp44,MAPK3,Microtubule-associatedprotein2kinase,MNK1,p44-ERK1,p44-MAPK)isencodedbytheMapk3(alsoknownasErk1,Prkm3)gene(GeneID50689)inratspecies.ERK1(a.k.a.MAPK3)andERK2(a.k.a.MAPK1orMAPK2)arerelatedserine/threoninekinasesoftheRas-Raf-MEK-ERKsignalingpathway.ERK1/2areactivatedbyMEK1/2-catalyzedphosphorylationattheirconservedThr-Glu-Tyr(TEY)dualphosphorylationmotif,firstattheTyrresidue(human/ratERK1Tyr204/Tyr203,human/ratERK2Tyr187/Tyr183)andthenattheThrresidue(human/ratERK1Thr202/Thr201,human/ratERK2Thr185/Thr181).ERK1/2areproline-directedkinasesthatpreferentiallycatalyzethephosphorylationofPro-Xxx-Ser/Thr-Prosequencemotifinhundredsofcytoplasmicandnuclearsubstrates,includingtranscriptionfactorssuchasEts,Elk,andc-Fos.Besidesthisprimarystructurerequirement,manyERK1/2substratespossessaD-dockingsiteand/oranF-dockingsite.Avarietyofscaffoldproteins,includingKSR1/2,IQGAP1,MP1,andβ-Arrestin1/2,alsoparticipateintheregulationofERK1/2MAPkinasecascade.Ras-Raf-MEK-ERKsignalingactivityisupregulatedinaboutone-thirdofallhumancancers,andtargetedinhibitionagainstcomponentsofthissignalingpathwayrepresentsapopularanticancerstrategy.
        ProductInformation
        FormatPurified
        Control
        • A431celllysate
        PresentationPurifiedrabbitpolyclonalinbuffercontaining0.1MTris-Glycine(pH7.4),150mMNaClwith0.05%sodiumazide.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat2-8°Cfromdateofreceipt.
        Applications
        ApplicationDetectMAPK3/MAPK1(ERK1/ERK2)usingthisAnti-MAPK1/2AntibodyvalidatedforuseinImmunocytochemistry,Immunoprecipitation,andWesternBlotting.
        KeyApplications
        • WesternBlotting
        • Immunoprecipitation
        • Immunocytochemistry
        ApplicationNotesWesternBlottingAnalysis:0.5μg/mLfromarepresentativelotdetectedMAPK3/MAPK1(ERK1/ERK2)inBALB/3T3,A431,andL6celllysates.



        WesternBlotting(SNAPID)Analysis:1-2μg/mLfromarepresentativelotdetectedMAPK3/MAPK1(ERK1/ERK2)inBALB/3T3,A431,andL6celllysates.



        ImmunocytochemistryAnalysis:A1:500dilutionfromarepresentativelotdetectedMAPK3/MAPK1(ERK1/ERK2)inNIH/3T3,A431,HeLa,andC2C12cells.
        BIOLOGicalInformation
        ImmunogenKLH-conjugatedlinearpeptidecorrespondingtotheC-terminalsequenceofratMAPK3(ERK-1).
        EpitopeC-terminal
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostRabbit
        SpecificityThisantibodyrecognizesMAPK3/MAPK1(ERK1/ERK2)C-terminalsequence.
        SpeciesReactivity
        • Rat
        • Mouse
        • Human
        SpeciesReactivityNoteReactswithhuman,mouse,andrat.Expectedtoreactwithbovineandcaninebasedon94%sequencehomology.
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        GeneSymbol
        • Erk1
        • Erk2
        • Mapk
        • Mapk1
        • Mapk3
        • Prkm1
        • Prkm3
        PurificationMethodProteinApurfied.
        UniProtNumber
        MolecularWeight~44/42kDaobserved.41.26/36.30kDa(humanMAPK1isoform1/2),41.14kDa(ratMAPK1),41.14kDa(mouseMAPK1),43.00/38.14kDa(humanMAPK3isoform1/2),42.95/45.64kDa(ratMAPK3isoform1/2),42.93kDa(mouseMAPK3)calculated(Met1removed).TargetsequenceisnotpresentinhumanMAPK3splicedisoform3(ERK1b).
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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