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    當前位置 : Millipore >>> Millipore/AB5220 | Anti-Growth Associated Protein-43 (GAP-43) Antibody/AB5220/100 µL
    Millipore/AB5220 | Anti-Growth Associated Protein-43 (GAP-43) Antibody/AB5220/100 µL
    • Millipore/AB5220 | Anti-Growth Associated Protein-43 (GAP-43) Antibody/AB5220/100 µL

    Millipore/AB5220 | Anti-Growth Associated Protein-43 (GAP-43) Antibody/AB5220/100 µL

    價格: ¥4680.00 市場價: 7800.00

    貨號: AB5220
    品牌: Millipore
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      • Description
        CatalogueNumberAB5220
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-GrowthAssociatedProtein-43(GAP-43)Antibody
        AlternateNames
        • Neuromodulin
        BackgroundInformationGAP-43,acommonMarkerofdifferentiatingneurons,isexpressedatelevatedlevelsbydevelopingorregeneratingneuronsduringaxongrowth.WhileGAP-43isanintegralmembraneproteinassociatedwiththecytoplasmicsurfaceofaxonalgrowthcones(andsynapses),itisabsentfromdendriticgrowthcones.Inadultbrain,GAP-43isfoundinhighconcentrationinpresynapticareaswherememoryformationisthoughttooccur,suchasfrontalcortex,limbicsystemandhippocampus.PhosphorylationofGAP-43byPKC(atSer41onhuman,mouse,rat,andbovineGAP-43orSer42onchickenandXenopusGAP-43)isspecificallycorrelatedwithcertainformsofsynapticplasticity.
        ProductInformation
        FormatPurified
        Control
        • Ratdorsalrootgangliontissuethathasbeensubjectedtoaspinalnerveligation,culturedneurons.
        PresentationPurifiedrabbitpolyclonalinbuffercontainingPBSwith0.01%sodiumazideasapreservative.
        StorageandShippingInformation
        StorageConditionsStableforupto6monthsat-20oCinundilutedaliquotsfromdateofreceipt.
        Avoidrepeatedfreeze/thawcycles.Duringshipment,smallvolumesofproductwilloccasionallybecomeentrappedinthesealoftheproductvial.Forproductswithvolumesof200μLorless,werecommendgentlytappingthevialonahardsurfaceorbrieflycentrifugingthevialinatabletopcentrifugetodislodgeanyliquidinthecontainer’scap.
        Applications
        ApplicationAnti-GrowthAssociatedProtein-43(GAP-43)AntibodyisanantibodyagainstGrowthAssociatedProtein-43(GAP-43)foruseinELISA,IC,IH,IP&WB.
        KeyApplications
        • ELISA
        • Immunocytochemistry
        • Immunohistochemistry
        • Immunoprecipitation
        • WesternBlotting
        ApplicationNotesImmunohistochemistry:
        1:1,000dilutionofapreviouslotwasusedon4%paraformaldehyde/PBSfixedor3%Paraformaldehyde/0.1%Gluteraldehyde/PBSfixedtissuesections.

        Immunocytochemistry:
        1:1,000dilutionofapreviouslotwasusedonHippocampalneuronsincultureandPC12cellsstimulatedwithNGF.

        Immunoprecipitation:
        1:100dilutionofapreviouslotwasused.

        ELISA:
        1:2,000dilutionofapreviouslotwasused.
        BIOLOGicalInformation
        ImmunogenRecombinantratGAP-43(completesequence).
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostRabbit
        SpecificityRecognizesGrowthAssociatedProtein-43(GAP-43).
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        • Chicken
        • Bovine
        SpeciesReactivityNoteReactstoahighdegreewithmouse.Reactstoalesserdegreewithrat,human,bovineandchicken.Otherspecieshavenotbeentested.
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryTheproteinencodedbythisgenehasbeentermeda"growth"or"plasticity"proteinbecauseitisexpressedathighlevelsinneuronalgrowthconesduringdevelopmentandaxonalregeneration.Thisencodedproteinisconsideredacrucialcomponentofaneffectiveregenerativeresponseinthenervoussystem.
        GeneSymbol
        • GAP43
        • PP46
        • B-50
        PurificationMethodAmmoniumSulfatePrecipitation
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P17677#Thisproteinisassociatedwithnervegrowth.Itisamajorcomponentofthemotilegrowthconesthatformthetipsofelongatingaxons.
        SIZE:238aminoacids;24803Da
        SUBUNIT:BindscalmodulinwithagreateraffinityintheabsenceofCa(2+)thaninitspresence(Bysimilarity).
        SUBCELLULARLOCATION:Cellmembrane;Peripheralmembraneprotein;Cytoplasmicside.Cellprojection,growthconemembrane;Peripheralmembraneprotein;Cytoplasmicside.Celljunction,synapse.Note=Cytoplasmicsurfaceofgrowthconeandsynapticplasmamembranes.
        PTM:PhosphorylationofthisproteinbyaproteinkinaseCisspecificallycorrelatedwithcertainformsofsynapticplasticity.
        SIMILARITY:SwissProt:P17677##Belongstotheneuromodulinfamily.&Contains1IQdomain.
        MolecularWeight23.6kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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