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    當前位置 ： Millipore >>> Millipore/AB3756 | Anti-Rad51 Antibody/AB3756/100 µg
    Millipore/AB3756 | Anti-Rad51 Antibody/AB3756/100 µg
    • Millipore/AB3756 | Anti-Rad51 Antibody/AB3756/100 µg

    Millipore/AB3756 | Anti-Rad51 Antibody/AB3756/100 µg

    價格: ￥4068.00 市場價: 6780.00
    打印產品信息
    貨號: AB3756
    品牌: Millipore
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      • Description
        CatalogueNumberAB3756
        ReplacesMABE515
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-Rad51Antibody
        ProductInformation
        FormatAffinityPurified
        PresentationAffinitypurifiedimmunoglobulin.Precipitatedantibodyinasolutionof50%saturatedammoniumsulfateandPBScontainingnopreservatives.
        StorageandShippingInformation
        StorageConditionsMaintainunopenedvialat-70Cforupto6months.Avoidrepeatedfreeze/thawcycles.

        Preparation&Use:Toreconstitutetheantibody,centrifugetheantibodyvialatmoderatespeed(5,000rpm)for5minutestopellettheprecipitatedantibodyproduct.Carefullyremovetheammoniumsulfate/PBSbuffersolutionanddiscard.Itisnotnecessarytoremovealloftheammoniumsulfate/PBSsolution:10μLofresidualammoniumsulfatesolutionwillnoteffecttheresUSPensionoftheantibody.Donotlettheproteinpelletdry,asseverelossofantibodyreactivitycanoccur.

        ResuspendtheantibodypelletinanysuitableBIOLOGicalbuffer,standardPBSorTBS(pH7.3-7.5)aretypical.Volumesrequiredarenotcriticalbutitissuggestedthatthefinalantibodyconcentrationbebetween0.1mg/mLand1.0mg/mL.Forexample,toachievea1mg/mLconcentrationwith50μgofprecipitatedantibody,theamountofbufferneededwouldbe50μL.

        Carefullyaddtheliquidbuffertothepellet.DONOTVORTEX.Mixbygentlestirringwithawidepipettiporgentlefinger-tapping.Lettheprecipitatedantibodyrehydratefor1hourat4-25C°priortouse.Smallparticlesofprecipitatedantibodythatfailtoresuspendarenormal.Vialsareoverfilledtocompensateforanylosses.

        Therehydratedantibodysolutionscanbestoredundilutedat2-8C°for2monthswithoutanysignificantlossofactivity.Note,thesolutionisnotsterile,thuscareshouldbetakenifproductisstoredat2-8C°.Forstorageat-20C°,theadditionofanequalvolumeofglycerolcanbeused,however,itisrecommendedthatACSgradeorhigherglycerolbeused,assignificantlossofactivitycanoccuriftheglycerolusedisnotofhighquality.Forfreezing,itisrecommendedthattherehydratedantibodysolutionbefurtherdiluted1:1witha2%BSA(fractionV,highest-gradeavailable)solutionmadewiththerehydrationbuffer.Theresulting1%BSA/antibodysolutioncanbealiquotedandstoredfrozenat-70C°forupto6months.Avoidrepeatedfreeze/thawcycles.
        Applications
        ApplicationDetectRad51withAnti-Rad51Antibody(RabbitPolyclonalAntibody),thathasbeenshowntoworkinWB,ICC&IHC.
        KeyApplications
        • WesternBlotting
        • Immunocytochemistry
        • Immunohistochemistry
        ApplicationNotesWesternblot:1:200-1:1,000usingECL.

        Immunohistochemistry:1:200-1:1,000.

        Immunocytochemistry:1:200-1:1,000.

        Optimalworkingdilutionsmustbedeterminedbytheenduser.

        SUGGESTEDPROTOCOLFORIMMUNOHISTOCHEMISTRY

        1.Dissecttissuesandfreezeondryice

        2.Cutonacryostat-10umslices

        3.Dryslidesatroomtemperaturefor30-90min

        4.FixwithcoldAcetone/Methanol(50/50)2min-theneitherprocessfurtherorstoreat-20°C.Alternativeisfixationwith4%PFAandtreatmentwithatrypsinsolution.(0.05%trypsin,incubation10minutesat37°C,followedby3washeswithPBS)

        5.Letairdry

        6.PBS5min

        7.Incubatein50mMammoniumchloride30min

        8.PBS5min

        9.Blockingserum30-45min

        10.Primaryantibody90min-dilution1:100-1:600

        11.Wash3timesfor5min

        12.Secondaryfluorescentconjug.antibody30min

        13.Wash3timesfor5minandmount
        BiologicalInformation
        ImmunogenSyntheticpeptide,aminoacids25-41ofRAD51.
        HostRabbit
        SpecificityRecognizesRAD51.Themolecularweightoftheproteinis36,963Daltons.
        SpeciesReactivity
        • Human
        • Mouse
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryTheproteinencodedbythisgeneisamemberoftheRAD51proteinfamily.RAD51familymembersarehighlysimilartobacterialRecAandSaccharomycescerevisiaeRad51,andareknowntobeinvolvedinthehomologousrecombinationandrepairofDNA.ThisproteincaninteractwiththessDNA-bindingproteinRPAandRAD52,anditisthoughttoplayrolesinhomologouspairingandstrandtransferofDNA.ThisproteinisalsofoundtointeractwithBRCA1andBRCA2,whichmaybeimportantforthecellularresponsetoDNAdamage.BRCA2isshowntoregulateboththeintracellularlocalizationandDNA-bindingABIlityofthisprotein.LossofthesecontrolsfollowingBRCA2inactivationmaybeakeyeventleADIngtogenomicinstabilityandtumOrigenesis.Twoalternativelysplicedtranscriptvariantsofthisgene,whichencodedistinctproteins,havebeenreported.TranscriptvariantsutilizingalternativepolyAsignalsexist.
        GeneSymbol
        • RAD51
        • RAD51L3
        • HsT16930
        • BRCC5
        • hRAD51
        • HRAD51
        • RECA
        • HsRad51
        • RAD51D
        • HsRAD51
        • RAD51A
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:Q06609#MayparticipateinacommonDNAdamageresponsepathwayassociatedwiththeactivationofhomologousrecombinationanddouble-strandbreakrepair.BindstosingleanddoublestrandedDNAandexhibitsDNA-dependentATPaseactivity.UnderwindsduplexDNAandformshelicalnucleoproteinfilaments.
        SIZE:339aminoacids;36966Da
        SUBUNIT:InteractswithBRCA1,BRCA2andeitherdirectlyorindirectlywithp53.InteractswithXRCC3,RAD54LandRAD54B.PartofacomplexwithRAD51CandRAD51B.InteractswithRAD51AP1.InteractswithCHEK1/CHK1,andthismayrequirepriorphosphorylationofCHEK1.
        SUBCELLULARLOCATION:Nucleus.Note=ColocalizeswithRAD51AP1tomultiplenuclearfociuponinductionofDNAdamage.
        TISSUESPECIFICITY:Highlyexpressedintestisandthymus,followedbysmallintestine,placenta,colon,pancreasandovary.Weaklyexpressedinbreast.
        PTM:Phosphorylated.PhosphorylationofThr-309byCHEK1/CHK1mayenhanceassociationwithchromatinatsitesofDNAdamageandpromoteDNArepairbyhomologousrecombination.DISEASE:SwissProt:Q06609#DefectsinRAD51areassociatedwithbreastcancer(BC)[MIM:114480].
        SIMILARITY:SwissProt:Q06609##BelongstotherecAfamily.RAD51subfamily.&Contains1HhHdomain.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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