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    當前位置 : Millipore >>> Millipore/AB1922 | Anti-Integrin β4 Antibody/AB1922/100 µL
    Millipore/AB1922 | Anti-Integrin β4 Antibody/AB1922/100 µL
    • Millipore/AB1922 | Anti-Integrin β4 Antibody/AB1922/100 µL

    Millipore/AB1922 | Anti-Integrin β4 Antibody/AB1922/100 µL

    價格: ¥4068.00 市場價: 6780.00

    貨號: AB1922
    品牌: Millipore
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      • Description
        CatalogueNumberAB1922
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-Integrinβ4Antibody
        AlternateNames
        • CD104
        ProductInformation
        FormatSerum
        PresentationWholerabbitserum.Liquid,with0.02%sodiumazide.
        StorageandShippingInformation
        StorageConditionsTheundilutedantibodysolutionisstableforapproximately6monthswhenstored-20°C.
        Applications
        ApplicationAnti-Integrinβ4AntibodyisanantibodyagainstIntegrinβ4foruseinIP,IH.
        KeyApplications
        • Immunoprecipitation
        • Immunohistochemistry
        ApplicationNotesImmunoprecipitationfromlabeledcellextracts:5μLofserumissufficienttoprecipitatealpha6beta4from1-2x10E6cells.Insomecasesimmunoprecipitationfrom35Smethioninelabeledcellsresultsintwobandsof95and55kDaofunknownorigininadditiontothe200kDaand140kDabandscorrespondingtobeta4andalpha6.

        Immunohistochemistry:5μg/mL.Seeprotocolbelow.

        Optimalworkingdilutionsmustbedeterminedbytheenduser.



        SuggestedProtocolforImmunohistochemical:

        1.Fixcryostatsectionswitha1:1chloroform-acetonemixturefor10minutesatroomtemperature.

        2.AirDry.

        3.Washwith2mLof1xPBS.

        4.Add200μLoffirstantibody(5μg/mL)toeachslide.Incubatefor1houratroomtemperatureinahumiditychamber.

        5.Washwith5mLof1xPBS.

        6.Addappropriatelydilutedanti-rabbitsecondaryantibodyconjugateandincubatefor1hourinahumiditychamber

        7.Washwith5mLof1xPBS.

        8.Add200μLofdevelopmentsolution(containingDABorothersubstratedependingonthesecondaryantibody)andincubateatroomtemperatureforthenecessarytimetodevelopthedarkcolor.Followthereactionwithamicroscope,toestablishthecorrectcolorintensity.

        9.Blockthereactionbywashingwith2mLof1xPBS.

        10.Counterstainwithhematoxylinasdesired.
        BIOLOGicalInformation
        ImmunogenSyntheticpeptidederivedfromtheCOOHterminalsequence(cytoplasmicdomain)ofthehumanβ4integrinsubunit(Tamuraetal.1990).ThesequenceusedisNH2-(K)GTLSTHMDQQFFQT-amide.Thelysine(K)isnotintheβ4sequenceandwasaddedtoconjugatethepeptid
        HostRabbit
        SpecificitySpecificforbeta4integrinasdeterminedbyimmunoprecipitation.Theepitoperecognizedisonthecytoplasmicdomainoftheproteinandisthusintracellular.Nocross-reactivitytootherintegrinbsubunits.
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryIntegrinsareheterodimerscomprisedofalphaandbetasubunits,thatarenoncovalentlyassociatedtransmembraneglycoproteinreceptors.Differentcombinationsofalphaandbetapolypeptidesformcomplexesthatvaryintheirligand-bindingspecificities.Integrinsmediatecell-matrixorcell-celladhesion,andtransducedsignalsthatregulategeneexpressionandcellgrowth.Thisgeneencodestheintegrinbeta4subunit,areceptorforthelaminins.Thissubunittendstoassociatewithalpha6subunitandislikelytoplayapivotalroleinthebiologyofinvasivecarcinoma.Mutationsinthisgeneareassociatedwithepidermolysisbullosawithpyloricatresia.Multiplealternativelysplicedtranscriptvariantsencodingdistinctisoformshavebeenfoundforthisgene.
        GeneSymbol
        • ITGB4
        • CD104
        • GP150
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P16144#Integrinalpha-6/beta-4isareceptorforlaminin.Itplaysacriticalstructuralroleinthehemidesmosomeofepithelialcells.
        SIZE:1822aminoacids;202151Da
        SUBUNIT:Heterodimerofanalphaandabetasubunit.Beta-4associateswithalpha-6.
        SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
        TISSUESPECIFICITY:Integrinalpha-6/beta-4ispredominantlyexpressedbyepithelia.Isoformbeta-4Disalsoexpressedincolonandplacenta.Isoformbeta-4Eisalsoexpressedinepidermis,lung,duodenum,heart,spleenandstomach.
        DOMAIN:SwissProt:P16144Thefibronectintype-III-likedomainsbindBPAG1andplectinandprobablyalsorecruitBP230.
        DISEASE:SwissProt:P16144#DefectsinITGB4areacauseofepidermolysisbullosaletaliswithpyloricatresia(EB-PA)[MIM:226730];alsoknownasjunctionalepidermolysisbullosawithpyloricatresia(PA-JEB)oraplasiacutiscongenitawithgastrointestinalatresia.EB-PAischaracterizedbymucocutaneousfragilityandgastrointestinalatresia,whichmostcommonlyaffectsthepylorus.&DefectsinITGB4areacauseofgeneralizedatrophicbenignepidermolysisbullosa(GABEB)[MIM:226650].Thisnonlethalformofjunctionalepidermolysisbullosaischaracterizedbylife-longblisteringoftheskin,associatedwithhairandtoothabnormalities.
        SIMILARITY:Belongstotheintegrinbetachainfamily.&Contains1Calx-betadomain.&Contains4fibronectintype-IIIdomains.&Contains1VWFAdomain.
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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