| Immunogen | SyntheticpeptidecorrespondingtotheC-terminusofthepredictedratVAChTprotein. |
| Host | GuineaPig |
| Specificity | VesicularAcetylcholineTransporter(VAChT).ByWesternblottheantibodyreactedwithasinglebandinratbrainhomogenates.ThisbandcorrespondedtothesizeofthepredictedrVAChT.Therewasnostainingofblotsincubatedwiththeantibodythathadbeenpreabsorbedwiththepeptideusedforantibodyproduction.Byimmunohistochemistrythestainingwasstrongintheinnerretinawherecholinergicprocessesarelocatedandweakincholinergiccellbodies.Therewasnobackgroundstainingnoted.IntheCNSstainingwasstronginfibersandstainscholinergiccellbodieswell. |
| SpeciesReactivity | |
| AntibodyType | PolyclonalAntibody |
| EntrezGeneNumber | |
| EntrezGeneSummary | Thisgeneisamemberofthevesicularaminetransporterfamily.Theencodedtransmembraneproteintransportsacetylcholineintosecretoryvesiclesforreleaseintotheextracellularspace.AcetylcholinetransportutilizesaprotongrADIentestablishedbyavacuolarATPase.Thisgeneislocatedwithinthefirstintronofthecholineacetyltransferasegene. |
| GeneSymbol | - SLC18A3
- MGC12716
- VACHT
- VAChT
|
| UniProtNumber | |
| UniProtSummary | FUNCTION:SwissProt:Q16572#Involvedinacetylcholinetransportintosynapticvesicles. SIZE:532aminoacids;56961Da SUBCELLULARLOCATION:Membrane;Multi-passmembraneprotein. TISSUESPECIFICITY:Peripheralandcentralcholinergicnervoussystems. SIMILARITY:SwissProt:Q16572##Belongstothemajorfacilitatorsuperfamily.Vesiculartransporterfamily. |