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    當前位置 : Millipore >>> Millipore/AB1530 | Anti-Peripherin Antibody/AB1530/100 µL
    Millipore/AB1530 | Anti-Peripherin Antibody/AB1530/100 µL
    • Millipore/AB1530 | Anti-Peripherin Antibody/AB1530/100 µL

    Millipore/AB1530 | Anti-Peripherin Antibody/AB1530/100 µL

    價格: ¥4188.00 市場價: 6980.00

    貨號: AB1530
    品牌: Millipore
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      • Description
        CatalogueNumberAB1530
        BrandFamilyChemicon®
        TradeName
        • Chemicon
        DescriptionAnti-PeripherinAntibody
        BackgroundInformationPeripherinisa56-58kDatypeIIIintermediatefilamentprotein(Portieretal.1983),expressedinmotor,sensoryandsympatheticsystemneuronsaswellasneuroendocrineskincarcinomasandcertainmelanocytictumors.Itisoftenusedanindicatorofentericganglioncelldensityinnormalanddiseased(e.g.Hirschsprung"sdisease)states.AbnormalinclusionscontainingperipherinhavealsobeenreportedinALS.Duringnormal,lateneurogenesisperipherinappearsaftertheexpressionofnestin,vimentin,alpha-internexinandneurofilamentsandisinvolvedinthedifferentiationofneurons,namelyoftheperipheralnervoussystem,andalsoprobablyinaxonalregeneration.Peripherinisnotrelatedtoperipherin-RDS,aphotoreceptorproteinassociatedwithretinaldegenerationandblindness.
        ProductInformation
        FormatSerum
        Control
        • Ratsensoryneurons,ratspinalcordhomogenateandperipheralnervehomogenate.
        PresentationRabbitpolyclonalantisera,nopreservatives.
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cinundilutedaliquotsfromdateofreceipt.
        HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
        Applications
        ApplicationThisAnti-PeripherinAntibodyisvalidatedforuseinIH,IH(P),WBforthedetectionofPeripherin.
        KeyApplications
        • Immunohistochemistry
        • Immunohistochemistry(Paraffin)
        • WesternBlotting
        ApplicationNotesAdditionalResearchApplications

        Immunohistochemistry:
        Apreviouslotofthisantibodywasusedat1:100-1:200dilution.Itissuggestedthattheantibodybeusedonfrozensectionsfixedinacetoneat-20°C.AB1530willworkontissuefixedforonehourorlessinfresh4%paraformaldehyde.Ithasbeenreportedthatthisantibodycanbeusedonparaffinembeddedtissuesections.SeeCell&TissueResearch(1997)288:11-23&EuropeanJ.Dermatology(1998)8:339-342.

        ElectronMicroscopy:
        ApreviouslotofthisantibodywasusedonElectronMicroscopy.

        Optimalworkingdilutionsandprotocolsmustbedeterminedbyenduser.
        BIOLOGicalInformation
        ImmunogenElectrophoreticallypuretrp-E-peripherinfusionprotein[Dev.BrainRes.(1990)57:235-248],containingallbutthe4Nterminalaminoacidsofratperipherin.FusionproteinpurifiedfrombacterialinclusionbodiesbyDEAE-cellulosechromatographyin6MureafollowedbypreparativeSDS-PAGE.
        ConcentrationPleaserefertotheCertificateofAnalysisforthelot-specificconcentration.
        HostRabbit
        SpecificityRecognizesPeripherin.Theantibodystainsa~57kDabandcleanlyandspecificallyanddoesnotstainvimentin,GFAP,alpha-internexinoranyoftheneurofilamentsubunits.Strongstainingonrat,mouse,human,pigandcowperipherin.Doesnotstainchicken,quailorothermoredistantlyrelatedspecieswhichappeartolackperipherin.
        SpeciesReactivity
        • Human
        • Rat
        • Mouse
        • Bovine
        • Pig
        SpeciesReactivityNoteStrongstainingonratandhuman.Cross-reactswithmouse,pig,andbovine.Otherspeciesnottested.
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryThisgeneencodesacytoskeletalproteinfoundinneuronsoftheperipheralnervoussystem.TheencodedproteinisatypeIIIintermediatefilamentproteinwithhomologytoothercytoskeletalproteinssuchasdesmin,andisadifferentproteinthattheperipherinfoundinphotoreceptors.Mutationsinthisgenehavebeenassociatedwithsusceptibilitytoamyotrophiclateralsclerosis.
        GeneSymbol
        • PRPH
        • Peripherin
        • PRPH1
        • peripherin
        • NEF4
        PurificationMethodUnpurified
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P41219#Class-IIIneuronalintermediatefilamentprotein.
        SIZE:470aminoacids;53651Da
        SIMILARITY:SwissProt:P41219##Belongstotheintermediatefilamentfamily.
        MolecularWeight56-58kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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