The antiserum was produced against synthesized peptide derived from human TUBA1/3/4 around the phosphorylation site of Tyr272.
Immunogen Range
238-287
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:40000
Specificity
TUBA1/3/4 (Phospho-Tyr272) Antibody detects endogenous levels of TUBA1/3/4 only when phosphorylated at Tyr272.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.