The antiserum was produced against synthesized peptide derived from human p16-INK4a around the phosphorylation site of Ser152.
Immunogen Range
107-156
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
p16-INK4a (Phospho-Ser152) Antibody detects endogenous levels of p16-INK4a only when phosphorylated at Ser152.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p16-INK4a (Phospho-Ser152) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with EPO 20U/ml 15', using p16-INK4a (Phospho-Ser152) Antibody. The lane on the right is blocked with the phospho peptide.