The antiserum was produced against synthesized peptide derived from human Bcl-6 around the phosphorylation site of Ser333.
Immunogen Range
299-348
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
Bcl-6 (Phospho-Ser333) Antibody detects endogenous levels of Bcl-6 only when phosphorylated at Ser333.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BCL6
Database Links
Gene ID: 604 Swiss-Prot #: P41182
OMIM #: 109565 Unigene #: Hs.478588
Target Modification
Phospho
Modification Sites
H:S333 M:S334 R:S334
Application Images
Image 1
Western blot analysis of lysates from COLO205 cells treated with insulin 0.01U/ml 15', using Bcl-6 (Phospho-Ser333) Antibody. The lane on the right is blocked with the phospho peptide.