The antiserum was produced against synthesized peptide derived from human PDGFRa around the phosphorylation site of Tyr849.
Immunogen Range
816-865
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
PDGFRa (Phospho-Tyr849) Antibody detects endogenous levels of PDGFRa only when phosphorylated at Tyr849.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PGFRA
Database Links
Gene ID: 5156 Swiss-Prot #: P16234
OMIM #: 173490/607685 Unigene #: Hs.74615
Target Modification
Phospho
Modification Sites
H:Y849 M:Y849 R:Y848
Application Images
Image 1
Western blot analysis of lysates from 293 cells, using PDGFRa (Phospho-Tyr849) Antibody. The lane on the right is blocked with the phospho peptide.