The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Ser75.
Immunogen Range
41-90
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
Src (Phospho-Ser75) Antibody detects endogenous levels of Src only when phosphorylated at Ser75.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SRC
Database Links
Gene ID: 6714 Swiss-Prot #: P12931
OMIM #: 190090 Unigene #: Hs.195659
Target Modification
Phospho
Modification Sites
H:S75 M:S74 R:S75
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using Src (Phospho-Ser75) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COLO205 cells treated with EGF 200ng/ml 30', using Src (Phospho-Ser75) Antibody. The lane on the right is blocked with the phospho peptide.