The antiserum was produced against synthesized peptide derived from human RIPK2 around the phosphorylation site of Ser176.
Immunogen Range
146-195
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Specificity
RIPK2 (Phospho-Ser176) Antibody detects endogenous levels of RIPK2 only when phosphorylated at Ser176.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RIPK2
Database Links
Gene ID: 8767 Swiss-Prot #: O43353
OMIM #: 603455 Unigene #: Hs.103755
Target Modification
Phospho
Modification Sites
H:S176 M:S176
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using RIPK2 (Phospho-Ser176) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with UV 15', using RIPK2 (Phospho-Ser176) Antibody. The lane on the right is blocked with the phospho peptide.