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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | MAP2K3 (Phospho-Thr222) Antibody |
| Synonyms | dual specificity mitogen-activated protein kinase kinase 3; kinase MKK3; MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3; MP2K3; PRKMK3 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MAP2K3 around the phosphorylation site of Thr222. |
| Immunogen Range | 188-237 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 |
| Specificity | MAP2K3 (Phospho-Thr222) Antibody detects endogenous levels of MAP2K3 only when phosphorylated at Thr222. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | MAP2K3 |
| Database Links |
Gene ID: 5606 Swiss-Prot #: P46734 OMIM #: 602315 Unigene #: Hs.514012 |
| Target Modification | Phospho |
| Modification Sites | H:T222 M:T222 R:T222 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Western blot analysis of lysates from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right is blocked with the phospho peptide. |
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