The antiserum was produced against synthesized peptide derived from human EPHA3/4/5 around the phosphorylation site of Tyr779/833.
Immunogen Range
746-795
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
Specificity
EPHA3/4/5 (Phospho-Tyr779/833) Antibody detects endogenous levels of EPHA3/4/5 only when phosphorylated at Tyr779/833.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human brain, using EPHA3/4/5 (Phospho-Tyr779/833) Antibody. The picture on the right is blocked with the phospho peptide.