The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Ser299.
Immunogen Range
266-315
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:10000
Specificity
A-RAF (Phospho-Ser299) Antibody detects endogenous levels of A-RAF only when phosphorylated at Ser299.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.