Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | AKT1/3(Phospho-Tyr437/434)Antibody |
Synonyms | RAC-PK-alpha;ProteinkinaseB;PKB;C-AKT |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanAKT1/3aroundthephosphorylationsiteofTyr437/434. |
ImmunogenRange | 406-455 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:20000 |
Specificity | AKT1/3(Phospho-Tyr437/434)AntibodydetectsendogenouslevelsofAKT1/3onlywhenphosphorylatedatTyr437/434. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | AKT1 |
DatabaseLinks | GeneID: 207/208/10000 Swiss-Prot#: P31749/Q9Y243 OMIM#: 164730/164731/611223 Unigene#: Hs.525622/Hs.631535/Hs.498292 |
TargetModification | Phospho |
ModificationSites | H:Y437/434M:Y437/434R:Y437/434 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanbrain,usingAKT1/3(Phospho-Tyr437/434)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromK562cellstreatedwithinsulin0.01U/ml15",usingAKT1/3(Phospho-Tyr437/434)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |