The antiserum was produced against synthesized peptide derived from human Caspase 8 around the phosphorylation site of Tyr380.
Immunogen Range
346-395
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Caspase 8 (Phospho-Tyr380) Antibody detects endogenous levels of Caspase 8 only when phosphorylated at Tyr380.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CASP8
Database Links
Gene ID: 841 Swiss-Prot #: Q14790
OMIM #: 211980/601763/607271 Unigene #: Hs.599762
Target Modification
Phospho
Modification Sites
H:Y380
Application Images
Image 1
Western blot analysis of lysates from LOVO cells treated with UV 15', using Caspase 8 (Phospho-Tyr380) Antibody. The lane on the right is blocked with the phospho peptide.