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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | ZAP-70 (Phospho-Tyr493) Antibody |
| Synonyms | 70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70; ZAP70; kinase ZAP70 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr493. |
| Immunogen Range | 460-509 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000 |
| Specificity | ZAP-70 (Phospho-Tyr493) Antibody detects endogenous levels of ZAP-70 only when phosphorylated at Tyr493. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | ZAP70 |
| Database Links |
Gene ID: 7535 Swiss-Prot #: P43403 OMIM #: 176947 Unigene #: Hs.234569 |
| Target Modification | Phospho |
| Modification Sites | H:Y493 M:Y492 R:Y488 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ZAP-70 (Phospho-Tyr493) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr493) Antibody. The lane on the right is blocked with the phospho peptide. |
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