The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Ser676.
Immunogen Range
643-692
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
Specificity
PKC thet (Phospho-Ser676) Antibody detects endogenous levels of PKC thet only when phosphorylated at Ser676.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PRKCQ
Database Links
Gene ID: 5588 Swiss-Prot #: Q04759
OMIM #: 600448 Unigene #: Hs.498570
Target Modification
Phospho
Modification Sites
H:S676 M:S676 R:S676
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC thet (Phospho-Ser676) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells treated with PMA 200nM 30', using PKC thet (Phospho-Ser676) Antibody. The lane on the right is blocked with the phospho peptide.