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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | MEK1/2 (Phospho-Ser217) Antibody |
| Synonyms | Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK1; MP2K1; PRKMK1; kinase MEK1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser217. |
| Immunogen Range | 189-238 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000 |
| Specificity | MEK1/2 (Phospho-Ser217) Antibody detects endogenous levels of MEK1/2 only when phosphorylated at Ser217. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | MAP2K2 |
| Database Links |
Gene ID: 5604/5605 Swiss-Prot #: Q02750/P36507 OMIM #: 115150/176872/115150/601263 Unigene #: Hs.145442/Hs.465627 |
| Target Modification | Phospho |
| Modification Sites | H:S218/222 M:S218/222 R:S218/222 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 (Phospho-Ser217) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using MEK1/2 (Phospho-Ser217) Antibody. The lane on the right is blocked with the phospho peptide. |
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