The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
MARCKS (Phospho-Ser158) Antibody
Synonyms
80K-L protein; MACS; MARCS; myristoylated alanine-rich C-kinase substrate; PKCSL; PRKCSL; protein kinase C substrate, 80 kDa protein, light chain
Immunogen
The antiserum was produced against synthesized peptide derived from human MARCKS around the phosphorylation site of Ser158.
Immunogen Range
126-175
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
MARCKS (Phospho-Ser158) Antibody detects endogenous levels of MARCKS only when phosphorylated at Ser158.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MARCKS
Database Links
Gene ID: 4082 Swiss-Prot #: P29966
OMIM #: 177061 Unigene #: Hs.519909/Hs.712721
Target Modification
Phospho
Modification Sites
H:S159 M:S152 R:S152
Application Images
Image 1
Western blot analysis of lysates from K562 cells treated with EGF 200ng/ml 30', using MARCKS (Phospho-Ser158) Antibody. The lane on the right is blocked with the phospho peptide.