The antiserum was produced against synthesized peptide derived from human Integrin beta3 around the phosphorylation site of Tyr773.
Immunogen Range
739-788
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
Integrin beta3 (Phospho-Tyr773) Antibody detects endogenous levels of Integrin beta3 only when phosphorylated at Tyr773.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ITGB3
Database Links
Gene ID: 3690 Swiss-Prot #: P05106
OMIM #: 173470/273800 Unigene #: Hs.218040
Target Modification
Phospho
Modification Sites
H:Y773 M:Y772 R:Y772
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Integrin beta3 (Phospho-Tyr773) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HL-60 cells treated with H2O2, using Integrin beta3 (Phospho-Tyr773) Antibody. The lane on the right is blocked with the phospho peptide.