Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
| ProductName | HNF4alpha(Phospho-Ser313)Antibody |
| Synonyms | HNF-4;HNF-4-alpha;HNF4;HNF4A;Hepatocytenuclearfactor4-alpha;NR2A1;TCF14;Transcriptionfactor14;TranscriptionfactorHNF-4 |
| Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanHNF4alphaaroundthephosphorylationsiteofSer313. |
| ImmunogenRange | 280-329 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:20000 |
| Specificity | HNF4alpha(Phospho-Ser313)AntibodydetectsendogenouslevelsofHNF4alphaonlywhenphosphorylatedatSer313. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | HNF4A |
| DatabaseLinks | GeneID: 3172 Swiss-Prot#: P41235 OMIM#: 125850/600281/606391 Unigene#: Hs.116462 |
| TargetModification | Phospho |
| ModificationSites | H:S313M:S313R:S313 |
| ApplicationImages |
| Image1 | ImmunofluorescenceanalysisofLOVOcells,usingHNF4alpha(Phospho-Ser313)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanlivercarcinoma,usingHNF4alpha(Phospho-Ser313)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image3 | WesternblotanalysisoflysatesfromHUVECcellstreatedwithEGF200ng/ml30",usingHNF4alpha(Phospho-Ser313)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |