HNF4 alpha (Phospho-Ser313) Antibody detects endogenous levels of HNF4 alpha only when phosphorylated at Ser313.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HNF4A
Database Links
Gene ID: 3172 Swiss-Prot #: P41235
OMIM #: 125850/600281/606391 Unigene #: Hs.116462
Target Modification
Phospho
Modification Sites
H:S313 M:S313 R:S313
Application Images
Image 1
Immunofluorescence analysis of LOVO cells, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using HNF4 alpha (Phospho-Ser313) Antibody. The lane on the right is blocked with the phospho peptide.