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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | HER2 (Phospho-Tyr1248) Antibody |
| Synonyms | C-erbB-2; Epidermal growth factor receptor-related protein; ErbB2; HER2; kinase ErbB2; MLN 19; NEU; NEU proto-oncogene; NGL; p185erbB2; Receptor protein-tyrosine kinase erbB-2; Receptor protein-tyrosine kinase erbB-2 precursor; Tyrosine kinase-type cell s |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1248. |
| Immunogen Range | 1206-1255 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 |
| Specificity | HER2 (Phospho-Tyr1248) Antibody detects endogenous levels of HER2 only when phosphorylated at Tyr1248. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | ERBB2 |
| Database Links |
Gene ID: 2064 Swiss-Prot #: P04626 OMIM #: 164870 Unigene #: Hs.446352 |
| Target Modification | Phospho |
| Modification Sites | H:Y1248 M:Y1249 R:Y1250 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr1248) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using HER2 (Phospho-Tyr1248) Antibody. The lane on the right is blocked with the phospho peptide. |
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