The antiserum was produced against synthesized peptide derived from human HDAC8 around the phosphorylation site of Ser39.
Immunogen Range
May-54
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity
HDAC8 (Phospho-Ser39) Antibody detects endogenous levels of HDAC8 only when phosphorylated at Ser39.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HDAC8
Database Links
Gene ID: 55869 Swiss-Prot #: Q9BY41
OMIM #: 300269 Unigene #: Hs.310536
Target Modification
Phospho
Modification Sites
H:S39 M:S39 R:S39
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using HDAC8 (Phospho-Ser39) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from NIH/3T3 cells, using HDAC8 (Phospho-Ser39) Antibody. The lane on the right is blocked with the phospho peptide.