The antiserum was produced against synthesized peptide derived from human GATA1 around the phosphorylation site of Ser142.
Immunogen Range
109-158
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
GATA1 (Phospho-Ser142) Antibody detects endogenous levels of GATA1 only when phosphorylated at Ser142.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
GATA1
Database Links
Gene ID: 2623 Swiss-Prot #: P15976
OMIM #: 300367/305371/314050 Unigene #: Hs.765
Target Modification
Phospho
Modification Sites
H:S142 M:S142 R:S142
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GATA1 (Phospho-Ser142) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from K562 cells, using GATA1 (Phospho-Ser142) Antibody. The lane on the right is blocked with the phospho peptide.