The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr397.
Immunogen Range
364-413
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
FAK (Phospho-Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr397.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PTK2
Database Links
Gene ID: 5747 Swiss-Prot #: Q05397
OMIM #: 600758 Unigene #: Hs.395482
Target Modification
Phospho
Modification Sites
H:Y397 M:Y397 R:Y397
Application Images
Image 1
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using FAK (Phospho-Tyr397) Antibody. The lane on the right is blocked with the phospho peptide.