The antiserum was produced against synthesized peptide derived from human p44/42 MAP Kinase around the phosphorylation site of Tyr204.
Immunogen Range
170-219
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
p44/42 MAP Kinase (Phospho-Tyr204) Antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Tyr204.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MAPK3
Database Links
Gene ID: 5595/5594 Swiss-Prot #: P27361/P28482
OMIM #: 601795/176948 Unigene #: Hs.861/Hs.431850
Target Modification
Phospho
Modification Sites
H:Y204/187 M:Y205/185 R:Y205/185
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p44/42 MAP Kinase (Phospho-Tyr204) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using p44/42 MAP Kinase (Phospho-Tyr204) Antibody. The lane on the right is blocked with the phospho peptide.