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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | eNOS (Phospho-Thr494) Antibody |
| Synonyms | cNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; Nitric-oxide synthase, endothelial; NOS, type III; NOS3; NOSIII |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Thr494. |
| Immunogen Range | 462-511 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 ELISA: 1:5000 |
| Specificity | eNOS (Phospho-Thr494) Antibody detects endogenous levels of eNOS only when phosphorylated at Thr494. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | NOS3 |
| Database Links |
Gene ID: 4846 Swiss-Prot #: P29474 OMIM #: 163729 Unigene #: Hs.647092 |
| Target Modification | Phospho |
| Modification Sites | H:T495 M:T494 R:T494 |
| Application Images | |
| Image 1 |
 Western blot analysis of lysates from Jurkat cells, using eNOS (Phospho-Thr494) Antibody. The lane on the right is blocked with the phospho peptide. |
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