The antiserum was produced against synthesized peptide derived from human Cofilin around the phosphorylation site of Ser3.
Immunogen Range
Jan-50
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
Cofilin (Phospho-Ser3) Antibody detects endogenous levels of Cofilin only when phosphorylated at Ser3.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CFL1
Database Links
Gene ID: 3716 Swiss-Prot #: P23528
OMIM #: 147795 Unigene #: Hs.207538
Target Modification
Phospho
Modification Sites
H:S3 M:S3 R:S3
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells treated with paclitaxel 1uM 24hours, using Cofilin (Phospho-Ser3) Antibody. The lane on the right is blocked with the phospho peptide.