The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
BAD (Phospho-Ser112) Antibody
Synonyms
BAD; BBC6; Bcl- XL/Bcl-2 associated death promoter; Bcl-2 binding component 6; Bcl-2-like 8 protein; Bcl-xL/Bcl-2 associated death promoter; Bcl2-antagonist of cell death; BCL2L8
Immunogen
The antiserum was produced against synthesized peptide derived from human BAD around the phosphorylation site of Ser112.
Immunogen Range
78-127
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
BAD (Phospho-Ser112) Antibody detects endogenous levels of BAD only when phosphorylated at Ser112.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BAD
Database Links
Gene ID: 572 Swiss-Prot #: Q92934
OMIM #: 603167 Unigene #: Hs.370254
Target Modification
Phospho
Modification Sites
H:S75 M:S112 R:S113
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using BAD (Phospho-Ser112) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with Forskolin, using BAD (Phospho-Ser112) Antibody. The lane on the left is blocked with the phospho peptide.