Description
SILACRPMI1640MediumisRPMI1640basalcellculturemediumwithoutL-arginine,L-glutamine,andL-lysineforuseduringSILACproteinlabelingwithstableisotope-labeledlysineand/orarginine.RPMI1640Mediumiswidelyusedforsupportingthegrowthofmanydifferentmammaliancells.CellssuccessfullyculturedinRPMI-1640includeHeLa,Jurkat,MCF-7,PC12,PBMC,astrocytes,andcarcinomas.Weofferavarietyof
Gibco™RPMI1640modificationsforarangeofapplications.Findtherightmodificationusingthemediaselectortool.
ThisRPMI1640Mediumismodifiedasfollows:| With | Without |
| | •Glucose |
| | •Phenolred |
| | •HEPES |
| | •L-arginine |
| | •L-glutamine |
RPMI1640Mediumisuniquefromothermediabecauseitcontainsthereducingagentglutathioneandhighconcentrationsofvitamins.RPMI1640Mediumcontainsbiotin,vitaminB12,andPABA,whicharenotfoundinEagle"sMinimalEssentialMediumorDulbecco"sModifiedEagleMedium.Inaddition,thevitaminsinositolandcholinearepresentinveryhighconcentrations.RPMI1640Mediumcontainsnoproteins,lipids,orgrowthfactors.Therefore,RPMI1640Mediumrequiressupplementation,commonlywith10%FetalBovineSerum(FBS).SILACproteinlabelingexperimentsshouldbeconductedusingGibco™DialyzedFBS.RPMI1640Mediumusesasodiumbicarbonatebuffersystem(2.0g/L),andthereforerequiresa5-10%CO
2environmenttomaintainphysiologicalpH.
cGMPManufacturingandQualitySystemSILACRPMI1640MediumismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ForResearchUseorFurtherManufacturing.Notforuseindiagnosticprocedures.