The antiserum was produced against synthesized peptide derived from human Proteasome alpha3 around the phosphorylation site of Ser250.
Immunogen Range
206-255
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
Proteasome alpha3 (Phospho-Ser250) Antibody detects endogenous levels of Proteasome alpha3 only when phosphorylated at Ser250.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PSMA3
Database Links
Gene ID: 5684 Swiss-Prot #: P25788
OMIM #: 176843/176845 Unigene #: Hs.558799
Target Modification
Phospho
Modification Sites
H:S250 M:S250 R:S250
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using Proteasome alpha3 (Phospho-Ser250) Antibody. The lane on the right is blocked with the phospho peptide.