The antiserum was produced against synthesized peptide derived from human MRE11 around the phosphorylation site of Ser264.
Immunogen Range
230-279
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:1000
Specificity
MRE11 (Phospho-Ser264) Antibody detects endogenous levels of MRE11 only when phosphorylated at Ser264.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
MRE11A
Database Links
Gene ID: 4361 Swiss-Prot #: P49959
OMIM #: 600814/604391 Unigene #: Hs.192649
Target Modification
Phospho
Modification Sites
H:S264 M:S264 R:S264
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells treated with forskolin 40nM 30', using MRE11 (Phospho-Ser264) Antibody. The lane on the right is blocked with the phospho peptide.