The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser304.
Immunogen Range
281-330
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at Ser304.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of lysates from COS7 cells treated with UV 15' and A549 cells, using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the right is blocked with the phospho peptide.