The antiserum was produced against synthesized peptide derived from human NEK9 around the phosphorylation site of Thr210.
Immunogen Range
176-225
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000
Specificity
NEK9 (Phospho-Thr210) Antibody detects endogenous levels of NEK9 only when phosphorylated at Thr210.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NEK9
Database Links
Gene ID: 91754 Swiss-Prot #: Q8TD19
OMIM #: 609798 Unigene #: Hs.7200
Target Modification
Phospho
Modification Sites
H:T210 M:T210 R:T209
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells, using NEK9 (Phospho-Thr210) Antibody. The lane on the right is blocked with the phospho peptide.