The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
AurB (Phospho-Tyr12) Antibody
Synonyms
AIK2; AIM-1; AIM1; ARK2; AURKB; Aurora- and Ipl1-like midbody-associated protein 1; Aurora-B; Aurora-related kinase 2; Aurora/IPL1-related kinase 2; Serine/threonine protein kinase 12; STK-1; STK12
Immunogen
The antiserum was produced against synthesized peptide derived from human AurB around the phosphorylation site of Tyr12.
Immunogen Range
Jan-50
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
AurB (Phospho-Tyr12) Antibody detects endogenous levels of AurB only when phosphorylated at Tyr12.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AURKB
Database Links
Gene ID: 9212 Swiss-Prot #: Q96GD4
OMIM #: 604970 Unigene #: Hs.442658
Target Modification
Phospho
Modification Sites
H:Y12 M:Y12 R:Y12
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using AurB (Phospho-Tyr12) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from K562 cells, using AurB (Phospho-Tyr12) Antibody. The lane on the right is blocked with the phospho peptide.