The antiserum was produced against synthesized peptide derived from human PAK1 around the phosphorylation site of Ser199.
Immunogen Range
165-214
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
PAK1 (Phospho-Ser199) Antibody detects endogenous levels of PAK1 only when phosphorylated at Ser199.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PAK1
Database Links
Gene ID: 5058 Swiss-Prot #: Q13153
OMIM #: 602590 Unigene #: Hs.435714
Target Modification
Phospho
Modification Sites
H:S199 M:S199 R:S198
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human placenta, using PAK1 (Phospho-Ser199) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from LOVO cells treated with starved 24h, using PAK1 (Phospho-Ser199) Antibody. The lane on the right is blocked with the phospho peptide.