Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | Kir5.1(Phospho-Ser417)Antibody |
Synonyms | IRK16;IRKG;InwardrectifierKchannelKir5.1;KCNJ16;PotassiumchannelinwardlyrectifyingsubfamilyJmember16 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfrommouseKir5.1aroundthephosphorylationsiteofSer417. |
ImmunogenRange | 369-418 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:5000 |
Specificity | Kir5.1(Phospho-Ser417)AntibodydetectsendogenouslevelsofKir5.1onlywhenphosphorylatedatSer417. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | KCNJ16 |
DatabaseLinks | GeneID: 16517 Swiss-Prot#: Q9Z307 Unigene#: Mm.30176 |
TargetModification | Phospho |
ModificationSites | M:S417R:S417 |
ApplicationImages |
Image1 | ImmunofluorescenceanalysisofHeLacells,usingKir5.1(Phospho-Ser417)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | Immunohistochemistryanalysisofparaffin-embeddedhumanbrain,usingKir5.1(Phospho-Ser417)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image3 | WesternblotanalysisoflysatesfromRAW264.7cellstreatedwithforskolin40nM30",usingKir5.1(Phospho-Ser417)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |