The antiserum was produced against synthesized peptide derived from human JAK3 around the phosphorylation site of Tyr785.
Immunogen Range
751-800
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
JAK3 (Phospho-Tyr785) Antibody detects endogenous levels of JAK3 only when phosphorylated at Tyr785.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
JAK3
Database Links
Gene ID: 3718 Swiss-Prot #: P52333
OMIM #: 600173/600802 Unigene #: Hs.515247
Target Modification
Phospho
Modification Sites
H:Y785 M:Y996 R:Y781
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using JAK3 (Phospho-Tyr785) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from Jurkat cells, using JAK3 (Phospho-Tyr785) Antibody. The lane on the right is blocked with the phospho peptide.