The antiserum was produced against synthesized peptide derived from human hnRNP K around the phosphorylation site of Ser284.
Immunogen Range
250-299
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
hnRNP K (Phospho-Ser284) Antibody detects endogenous levels of hnRNP K only when phosphorylated at Ser284.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HNRNPK
Database Links
Gene ID: 3190 Swiss-Prot #: P61978
OMIM #: 600712 Unigene #: Hs.522257
Target Modification
Phospho
Modification Sites
H:S284 M:S284 R:S284
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using hnRNP K (Phospho-Ser284) Antibody. The lane on the right is blocked with the phospho peptide.