The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
CDK9 (Phospho-Thr186) Antibody
Synonyms
C-2K; PITALRE; kinase Cdk9
Immunogen
The antiserum was produced against synthesized peptide derived from human CDK9 around the phosphorylation site of Thr186.
Immunogen Range
152-201
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
CDK9 (Phospho-Thr186) Antibody detects endogenous levels of CDK9 only when phosphorylated at Thr186.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDK9
Database Links
Gene ID: 1025 Swiss-Prot #: P50750
OMIM #: 603251 Unigene #: Hs.150423/Hs.706809
Target Modification
Phospho
Modification Sites
H:T186 M:T186 R:T186
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells treated with Forskolin 40nM 30', using CDK9 (Phospho-Thr186) Antibody. The lane on the right is blocked with the phospho peptide.