AurB (Phospho-Thr232) Antibody detects endogenous levels of AurB only when phosphorylated at Thr232.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
AURKB
Database Links
Gene ID: 9212 Swiss-Prot #: Q96GD4
OMIM #: 604970 Unigene #: Hs.442658
Target Modification
Phospho
Modification Sites
H:T232 M:T237 R:T235
Application Images
Image 1
Immunofluorescence analysis of HepG2 cells, using AurB (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using AurB (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from COS7 cells treated with Nocodazole 1ug/ml 16h, using AurB (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.