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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | HER3 (Phospho-Tyr1222) Antibody |
| Synonyms | c-erbB3; ERB3; ERBB3; HER3; kinase ErbB3; Receptor protein-tyrosine kinase erbB-3 precursor; Tyrosine kinase-type cell surface receptor HER3 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HER3 around the phosphorylation site of Tyr1222. |
| Immunogen Range | 1191-1240 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 |
| Specificity | HER3 (Phospho-Tyr1222) Antibody detects endogenous levels of HER3 only when phosphorylated at Tyr1222. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | ERBB3 |
| Database Links |
Gene ID: 2065 Swiss-Prot #: P21860 OMIM #: 190151/607598 Unigene #: Hs.118681 |
| Target Modification | Phospho |
| Modification Sites | H:Y1222 H:Y1219 H:Y1219 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of HUVEC cells, using HER3 (Phospho-Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human brain, using HER3 (Phospho-Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 3 |
 Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using HER3 (Phospho-Tyr1222) Antibody. The lane on the right is blocked with the phospho peptide. |
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