The antiserum was produced against synthesized peptide derived from human HCK around the phosphorylation site of Tyr410.
Immunogen Range
381-430
Clonality
Polyclonal
Application Dilutions
IF: 1:100~1:500 ELISA: 1:20000
Specificity
HCK (Phospho-Tyr410) Antibody detects endogenous levels of HCK only when phosphorylated at Tyr410.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
HCK
Database Links
Gene ID: 3055 Swiss-Prot #: P08631
OMIM #: 142370 Unigene #: Hs.655210
Target Modification
Phospho
Modification Sites
H:Y410 M:Y408 R:Y409
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using HCK (Phospho-Tyr410) Antibody. The picture on the right is blocked with the phospho peptide.