The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody
Synonyms
D1PAS1 related sequence 2, D1PAS1-RS2, DBX, DDX3X, DEAD-box RNA helicase DEAD3, DEAD-box protein 3, DEAD-box, X isoform, DEAD3, ERH, Embryonic RNA helicase, HLP2, Helicase-like protein 2
Immunogen
The antiserum was produced against synthesized peptide derived from human DDX3/DEAD-box Protein 3 around the phosphorylation site of Thr322.
Immunogen Range
466-515
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
Specificity
DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody detects endogenous levels of DDX3/DEAD-box Protein 3 only when phosphorylated at Thr322.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
DDX3X
Database Links
Gene ID: 1654 Swiss-Prot #: O00571
OMIM #: 300160 Unigene #: Hs.380774
Target Modification
Phospho
Modification Sites
H:T322 M:T322 R:T323
Application Images
Image 1
Immunofluorescence analysis of HUVEC cells treated with serum 20% 30', using DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human brain, using DDX3/DEAD-box Protein 3 (Phospho-Thr322) Antibody. The picture on the right is blocked with the phospho peptide.