The antiserum was produced against synthesized peptide derived from human CRYAB/Crystallin-alpha-B around the phosphorylation site of Ser59.
Immunogen Range
31-80
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:5000
Specificity
CRYAB/Crystallin-alpha-B (Phospho-Ser59) Antibody detects endogenous levels of CRYAB/Crystallin-alpha-B only when phosphorylated at Ser59.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CRYAB
Database Links
Gene ID: 1410 Swiss-Prot #: P02511
OMIM #: 123590/608810 Unigene #: Hs.408767
Target Modification
Phospho
Modification Sites
H:S59 M:S59 R:S59
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using CRYAB/Crystallin-alpha-B (Phospho-Ser59) Antibody. The picture on the right is blocked with the phospho peptide.