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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | 53BP1 (Phospho-Ser25) Antibody |
| Synonyms | p53-binding protein 1; P531; TP53B; TP53BP1; TRP53BP1; Tumor suppressor p53-binding protein 1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 53BP1 around the phosphorylation site of Ser25. |
| Immunogen Range | Oct-59 |
| Clonality | Polyclonal |
| Application Dilutions | IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 |
| Specificity | 53BP1 (Phospho-Ser25) Antibody detects endogenous levels of 53BP1 only when phosphorylated at Ser25. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | TP53BP1 |
| Database Links |
Gene ID: 7158 Swiss-Prot #: Q12888 OMIM #: 605230 Unigene #: Hs.440968 |
| Target Modification | Phospho |
| Modification Sites | H:S25 M:S25 R:S30 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 53BP1 (Phospho-Ser25) Antibody. The picture on the right is blocked with the phospho peptide. |
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